Road I/68 Ostrava-Hrušov (D1) - Havířov (I/11), Search Study

Import 07/02/2011Obsahem diplomové práce je vypracování vyhledávací studie vedení silnice I/68 v úseku Ostrava - Hrušov až Havířov. Práce je zpracována ve třech variantách. Ve variantě A s minimálním dopadem na životní prostředí a přírodu, ve variantě B s minimálním zásahem do zástavby a ve variantě C která prochází přesně středem vymezeného koridoru. Všechny návrhové parametry byly stanoveny dle norem a předpisů. V závěru je provedeno vyhodnocení variant. Vybrána nejvhodnější varianta a k ní rozpracovaná MÚK v obci Petřvald.The content of this thesis is to develop research study of the road I/68 in the section of Ostrava - Hrušov to Haviřov. The work is developed in three variants. In Option A, with minimal impact on the environment and nature, in variant B with minimal interference in buildings and in variant C, which passes through the exact center of the defined corridor. All design parameters were set according to standards and regulations. In conclusion, an evaluation of alternatives. Chosen the best option if it unfinished flyover in the town Petřvald.Prezenční227 - Katedra dopravního stavitelstvívýborn

Importance of morphological, ultrastructural, and molecular analyses for the higher taxonomy of ciliates

Ciliophora are one of the largest groups of protists. They have three typical features - (1). the presence of many cilia, covering the cell, (2). nuclear dualism, and (3). conjugation. The systematics of the phylum Ciliophora has changed greatly during the last decades. This was caused by the development of new methods used for studying the ciliates. The first systems were based on the morphology of structures in the oral region. Based on these structures, Levine et al. published a system of ciliates in 1980, which they divided into three classes. However, it was found later that this system was not natural because oral structures do not reflect the phylogeny, and the structure of somatic structures was shown to be a more useful feature. Small and Lynn created a system based on the structure of somatic kinetids, where they divided the ciliates into three subphyla and eight classes. However, subsequent molecular analyses did not support this system. They divided the ciliates into two subphyla (Postciliodesmatophora and Intramacronucleata), and the number of classes was elevated to eleven. Today, additional six new classes of ciliates are recognised. They were created mainly on the based of phylogenomic and phylogenetic analyses. The classes of the subphylum Intramacronucleata are grouped into three...Nálevníci jsou jednou z největších skupin protist. Mají tři charakteristické vlastnosti - (1). přítomnost mnoha bičíků, jimiž je pokryta buňka, (2). jaderný dualismus a (3). konjugaci. Systematika kmene Ciliophora se od jeho založení velmi změnila. To zapříčinil rozvoj nových metod, kterými se nálevníci mohli studovat. První systémy byly založeny na základě morfologie struktur v orální oblasti. Na základě těchto struktur vydali Levine a kolektiv v roce 1980 systém nálevníků, v kterém rozdělili nálevníky do tří tříd. Zjistilo se, že tento systém není přirozený, jelikož orální struktury jsou variabilní. Jako lepší znak pro evoluci nálevníků se osvědčila somatická ultrastruktura. Systém na základě ní vydali Small a Lynn, kteří vytvořili v kmenu Ciliophora tři podkmeny a osm tříd. Molekulární analýzy tento systém nepotvrdily. Na základě nich se ukázala existence pouze dvou podkmenů (Postciliodesmatophora a Intramacronucleata) a počet tříd se zvýšil na jedenáct. Od té doby bylo definováno dalších 6 nových tříd nálevníků. Tyto třídy byly vytvořeny především na základě fylogenomických a fylogenetických analýz. V podkmenu Intramacronucleata byly pomocí fylogenomických analýz odhaleny tři evoluční linie SAL, CONthreeP a Protocruziea. Klíčová slova: nálevník, systém, orální ciliatura, somatická ciliatura,...Department of ZoologyKatedra zoologiePřírodovědecká fakultaFaculty of Scienc

The role of iron in the virulence of Acanthamoeba castellanii

Iron is an essential nutrient for all living organisms. It plays a crucial role in the virulence of pathogenic bacteria as well as parasitic eukaryotes. Although iron is an abundant element in the host organism, it cannot be easily obtained by the parasite. The ability of parasites to induce disease depends largely on how efficient the mechanisms for iron acquisition have evolved. This thesis deals with the effect of iron on the virulence of Acanthamoeba castellanii. It is a free-living amoeba that, in exceptional situations, can cause fatal inflammatory brain disease or damage a person's vision. Culturing with mammalian cells allowed us to demonstrate the effect of iron on amoeba virulence using flow cytometry. Based on fluorescent labeling, we localized the protein AC_IDIP (Iron-Deprivation Induced Protein) in the cytosol. This protein was also isolated and a polyclonal antibody was raised against it and used to monitor the effect of iron availability on the AC_IDIP level. Using comparative proteomic analysis, it was possible to monitor changes in the A. castellanii proteome depending on the nutrient source. Altered levels of many proteins were detected under conditions where mammalian cells were the nutrient source. These were mainly cytoskeletal proteins. In contrast, the proportion of proteins...Železo je nezbytná živina pro všechny živé organismy. Má zásadní úlohu ve virulenci patogenních bakterií i parazitických eukaryot. I když je železo hojným prvkem v organismu hostitele, není pro parazita snadné ho získat. Schopnost parazitů vyvolat onemocnění závisí z velké části na tom, jak účinné mechanismy pro zisk železa vyvinuli. Tato práce se zabývá vlivem železa na virulenci Acanthamoeba castellanii. Je to volně žijící améba, která za výjimečných situací může u člověka vyvolat smrtelné zánětlivé onemocnění mozku nebo člověku poškodit zrak. Kultivací se savčími buňkami se nám povedlo prokázat vliv železa na virulenci améby za využití průtokové cytometrie. Na základě fluorescenčního značení jsme lokalizovali protein AC_IDIP (Iron-Deprivation Induced Protein) v cytosolu. Tento protein byl také izolován a byla proti němu vytvořena polyklonální protilátka, která byla použita ke sledování vlivu dostupnosti železa na hladinu AC_IDIP. Pomocí komparativní proteomické analýzy jsme byli schopni sledovat změny v proteomu A. castellanii v závislosti na zdroji živin. Identifikovali jsme velké množství proteinů, jež měly změněnou hladinu za podmínky, kde zdrojem živin byly savčí buňky. Jednalo se zejména o cytoskeletální proteiny. Oproti tomu zastoupení proteinů obsahujících železo, nebo se na jeho...Department of ParasitologyKatedra parazitologieFaculty of SciencePřírodovědecká fakult

Study of Unused Railyards SŽDC Ostrava

Import 01/09/2009Prezenční227 - Katedra dopravního stavitelstvívelmi dobř

Importance of morphological, ultrastructural, and molecular analyses for the higher taxonomy of ciliates

Ciliophora are one of the largest groups of protists. They have three typical features - (1). the presence of many cilia, covering the cell, (2). nuclear dualism, and (3). conjugation. The systematics of the phylum Ciliophora has changed greatly during the last decades. This was caused by the development of new methods used for studying the ciliates. The first systems were based on the morphology of structures in the oral region. Based on these structures, Levine et al. published a system of ciliates in 1980, which they divided into three classes. However, it was found later that this system was not natural because oral structures do not reflect the phylogeny, and the structure of somatic structures was shown to be a more useful feature. Small and Lynn created a system based on the structure of somatic kinetids, where they divided the ciliates into three subphyla and eight classes. However, subsequent molecular analyses did not support this system. They divided the ciliates into two subphyla (Postciliodesmatophora and Intramacronucleata), and the number of classes was elevated to eleven. Today, additional six new classes of ciliates are recognised. They were created mainly on the based of phylogenomic and phylogenetic analyses. The classes of the subphylum Intramacronucleata are grouped into three..

The role of iron in the virulence of Acanthamoeba castellanii

Iron is an essential nutrient for all living organisms. It plays a crucial role in the virulence of pathogenic bacteria as well as parasitic eukaryotes. Although iron is an abundant element in the host organism, it cannot be easily obtained by the parasite. The ability of parasites to induce disease depends largely on how efficient the mechanisms for iron acquisition have evolved. This thesis deals with the effect of iron on the virulence of Acanthamoeba castellanii. It is a free-living amoeba that, in exceptional situations, can cause fatal inflammatory brain disease or damage a person's vision. Culturing with mammalian cells allowed us to demonstrate the effect of iron on amoeba virulence using flow cytometry. Based on fluorescent labeling, we localized the protein AC_IDIP (Iron-Deprivation Induced Protein) in the cytosol. This protein was also isolated and a polyclonal antibody was raised against it and used to monitor the effect of iron availability on the AC_IDIP level. Using comparative proteomic analysis, it was possible to monitor changes in the A. castellanii proteome depending on the nutrient source. Altered levels of many proteins were detected under conditions where mammalian cells were the nutrient source. These were mainly cytoskeletal proteins. In contrast, the proportion of proteins..